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Macrogen offers primer walking service to confirm the sequence integrity of your clone or PCR products. We can perform end sequencing with the primers that you provide or use the reference sequence, if applicable, and design internal primers to fill in the gap.
1Kb single contig : 5~6 days.
  (Time consuming would vary based on the following reasons; primer synthesis and sequencing
    failure)
Phred score of 20 or higher quality contig is requirerd.
Time can be saved when primers are synthesized bidirectionally.
  (refer to the figure as follows)
 
 
Starting with the end sequencing product by using the primer provided or designed,
  internal primer will be designed and processed.
 
Using a new primer (but with the same template), internal primer continues reading at
  the appropriate location.
 
Each walking requires approximately 5~6 days; enabling extension of 500bp~600bp per direction.
 

Number of primers and sequence used for primer walking

Abi file used for primer walking
Contig sequence and contig quality