Home > Services > Sequencing service > Microbial Genome Full Sequencing
 
 
 
The genome base sequence analysis of Macrogen's Genome Business Division is largely classified into
3 parts:


Part I
- 2~5kb random shotgun library construction
- 10kb Insert Plasmid Library (Random Sheared Insert or Partially Digested Insert)
- Sequencing through DNA Analyzer 3730xl
- Assembly using ARACHNE
- Gap filling
 
Part II
- Gene Annotation (characterization of the predicted ORF and prediction of Structural RNA)
  a. BlastX Non redundant protein databases
  b. Cluster of Orthologous Group (NCBI)
  c. pFAM Protein families database of alignments and HMMs
  d. tRNA scan SE tRNA finding program (WashU)
 
Part III
- Provision of Genome Browser (Genome Browser is implemented to enable the client to check
  the complete results of the experiment and customized according to the clientĄ¯s request)