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DNA Sources
   Library Sources
  - Mammalian
     - Insert Preparation : Random Shearing or Partial Digestion
  - Plant
     - Insert Size : 1.5~10kb Insert plasmid library (Insert Size Std : 0.5~1kb)
  - Bacteria
     - Vector : High Copy number Vector or Low Copy number Vector
  - Virus
     - Library format : Cell or DNA
  - Bacteriophage
      
  - BAC DNA
   Time Required
  - Cosmid DNA
     - 4~5 weeks after checking whether each sample is normal
  - Fosmid DNA      

 
Step 1 : DNA preparation
  a.
b.


Genomic Shotgun Library: Separates high molecular weight DNA (100~400kb) from the host.
Large Construct Shotgun Library (BAC, Cosmid, Fosmid, Plasmid, etc.): Separates the sample DNA from the host and removes the host Genomic DNA. Checks the samples based on the size of the prepared sample DNA and the results of End Sequencing.

   
Step 2 : Insert Preparation 1
a.


b.

Random Shearing Method: Secures the insert with the desired size by size-selecting DNA(1~6kb) obtained through Nebulizer-based random shearing using Agarose gel electrophoresis once again
Partial Digestion: Secures the insert with the desired size by size-selecting the samples prepared through Sau3AI-based partial digestion using Agarose gel electrophoresis.
       
Step 3 : Insert Preparation 2
  Processes the insert to be ligated to the vector appropriately (blunting or CIP process).
   
Step 4 : Ligation
  Checks the quality of the library by ligating it to High Copy Vector or Low Copy Vector.
 
Library Report


Insert Size (Std 1kb<)
  Insert Ratio (95%>)
  TEST Sequencing Result
   
Provision of Library
  Provision of Cell or DNA