Home > Services > Library Construction Service > cDNA Library Construction
 
 
DNA Sources
   Formats
  - Mammalian
     - Standard cDNA Library
  - Plant
     - Normalized cDNA Library
 
    - Average Insert Size : 1.5~2.5kb
Time Required
     - Insert Size Range : 0.5~4.5kb
  - 4~6 weeks after checking     - Total Number of Primary Clones : 1.0X107
    whether each sample is normal      

Step 1 : Total RNA preparation
  Separates Cytoplasmic Total RNA.
   
Step 2 : Synthesize cDNA
Synthesizes First-strand cDNA through the Oligo (dT) linker-primer and reverse transcriptase; synthesizes Second-strand cDNA using DNA polymerase I. Ligates an appropriate adaptor.
* Optional: Normalization (Removes cDNA of higher frequency)
       
Step 3 : Size Selection and Cloning
  Ligates to an appropriate vector after size fraction through gel filtration.
   
Step 4 : Analysis
  - Redundancy check
- Blast Service
- GO (Gene Ontology Report)
- Others
 
Library Report


Average Insert Size check
  Insert Size Range Check
  TEST Analysis : Redundancy check and Blast Service
   
Provision of Library
  Provision of Phagemid or Cell or DNA
   
Analysis Report
  Redundancy check
  Blast Service
  GO (Gene Ontology Report)
  Others